Cultivation of normal human epidermal melanocytes in the absence of phorbol esters.
نویسندگان
چکیده
منابع مشابه
جداسازی و کشت اولیه ملانوسیت‌های پوست انسان و مقایسه‌ی کشت با و بدون فربول‌استر
Background: Primary culture takes place following the cell isolation from tissues. Isolation and culture of melanocytes based on their roll in the protection of body against hazardous sun rays, production of skin, cornea and hair color is really important. This study was done to set isolation, culture and proliferation of melanocytes from children foreskin and adult eyelashes, and also comparis...
متن کاملStudies of human uveal melanocytes in vitro: isolation, purification and cultivation of human uveal melanocytes.
PURPOSE To develop the methods for isolation and cultivation of human uveal melanocytes (UM) from adult donor eyes. METHODS After removal of the pigment epithelium, the uvea was pretreated in trypsin solution at 4 degrees C overnight, incubated at 37 degrees C with trypsin for 1 hr, then incubated with collagenase for 3 hr. Released cells were collected each hour during the incubation and cul...
متن کاملGrowth of human melanocyte cultures supported by 12-O-tetradecanoylphorbol-13-acetate is mediated through protein kinase C activation.
To investigate the role of protein kinase C (PKC) in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-dependent growth of human melanocytes, we analyzed the effects of phorbol ester treatment on both PKC expression and growth control in these cells. We found that established cultures of normal melanocytes contain the PKC alpha, PKC beta, and PKC epsilon isoforms. The abilities of various phorbol ...
متن کاملIsolation and Cultivation of Adult Human Keratinocyte Stem Cells for Regeneration of Epidermal Sheets
Background: Keratinocyte stem cell is one of the adult stem cells that inhabits the skin and contributes to skin function and renewal. Adult stem cells are best defined by their capacity to self-renew, and to maintain tissue function for a long period of time. These findings indicate the importance of these cells for clinical applications including regenerative medicine, tissue engineering and ...
متن کاملPigmentation and inhibition of wound contraction by cultured skin substitutes with adult melanocytes after transplantation to athymic mice.
Wound closure with cultured skin substitutes results in epithelium that is consistently hypopigmented. Hypothetically, addition of human melanocytes to cultured skin grafts may result in normal pigmentation of healed skin. Skin substitutes were composed of human epidermal keratinocytes and melanocytes, dermal fibroblasts, and collagen-glycosaminoglycan substrates, and were incubated for 12 d in...
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ورودعنوان ژورنال:
- Methods in molecular medicine
دوره 107 شماره
صفحات -
تاریخ انتشار 2005